Wanda K. Neu
Professor and Director of Undergraduate Studies
Electroporation-Mediated Drug and Gene Delivery
Electroporation refers to the creation of small (1-40 nm) pores in the lipid bilayer of the membrane in response to large electric shocks. Electroporation occurs as an undesirable side effect in accidental contacts with high voltage wires or when defibrillation shocks are applied to the heart to prevent sudden cardiac death. However, electroporation has also important applications in biotechnology, as pores allow the introduction of drugs and DNA into cells. Still, this technique is largely empirical and the results are often variable and difficult to control. Our research in this area concentrates on the development of a model of electroporation that would allow theoretical investigation of the creation of pores during the shock, the flow of ions, drugs, and DNA through pores, and the resulting changes in intracellular concentrations.
Nonlinear Dynamics Approach to Cardiac Arrhythmias
Cardiac arrhythmias, serious and potentially fatal diseases, can only rarely be successfully controlled with medication. The only available treatment is defibrillation, the delivery of a large electric shock. Such shocks often have serious side effects. Our research investigates the feasibility of alternative treatments for cardiac tachyarrhythmias that instead of large shocks use an appropriately timed train of small pulses. The approach is based on the feedback protocols that have been developed to control the dynamics of complex nonlinear systems and involves animal experiments, mathematical modeling, and computer simulations. These studies allow us to gain a better insight into the dynamics of cardiac rhythm and to assess the feasibility of bringing an episode of arrhythmia under control, terminate it, or even inhibit its occurrence.
- Office Location: 1151 CIEMAS
- Office Phone: (919) 660-5105
- Email Address:
- Web Page:
Research Interests: Electroporation-mediated drug delivery and gene therapy; Control of cardiac arrhythmias using nonlinear dynamics
- BME 560.01 - MOLEC BASIS MEMBRANE TRANSPORT
Representative Publications: (More Publications)
- Caroline L. Ring, David G. Schaeffer, Wanda Krassowska Neu, Effect of strength-interval relationship on cardiac rhythm dynamics in a one-dimensional mapping model (2011) (Dynamics Days '11, 30th Annual International Conference on Chaos and Nonlinear Dynamics, Chapel Hill, NC, Jan. 5-8.) .
- J. P. Cranford, W. Krassowska Neu, Electroporation in a three-dimensional, time-dependent model of a skeletal muscle fiber (2011) (Dynamics Days '11, 30th Annual International Conference on Chaos and Nonlinear Dynamics, Chapel Hill, NC, Jan. 5-8.) .
- Wanda Krassowska Neu, John C. Neu, Mechanism of irreversible electroporation in cells: Insight from the models (2010), pp. 85-122.
- L. Guo, J. C. Neu, J. P. Cranford, W. Krassowska Neu, Activating function of needle electrodes in anisotropic tissue, Med. Biol. Eng. Comput., vol 47 (2009), pp. 1001-1011.
- H.M. Dobrovolny, C. Berger, N.H. Brown, W. Krassowska Neu, D.J. Gauthier, Spatial heterogeneity of restitution properties and the onset of alternans (2009) (31st Annual International IEEE EMBS Conference of the IEEE Engineering in Medicine and Biology Society, Minneapolis, Minnesota, September, 2-6.) .